Reduced DNA repair in mouse satellite DNA after treatment with methyl-methanesulfonate, and N-methyl-N-nitrosourea
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چکیده
منابع مشابه
Nonuniform distribution of DNA repair in chromatin after treatment with methyl methanesulfonate.
The distribution of methyl methanesulfonate induced DNA repair was measured in mouse mammary cell chromatin by digestion of "repair labeled" nuclei with micrococcal nuclease. The results indicate that there is a nonuniform distribution of DNA repair in chromatin. The chromatin fraction digested during the first 5 minutes of incubation with micrococcal nuclease appears to be a primary site of DN...
متن کاملInhibition of N-methyl-N-nitrosourea-induced mutagenicity and DNA methylation by ellagic acid.
Ellagic acid, a naturally occurring plant phenol, inhibits the activity of the direct-acting mutagen N-methyl-N-nitrosourea (MeNU) in Salmonella typhimurium TA100. Ellagic acid at 0.10, 0.25, 0.50, and 1.00 mM inhibited the mutagenicity of MeNU (0.40 mM) by 3%, 13%, 45%, and 60%, respectively. Ellagic acid (3 mM) also inhibited the mutagenic activity of N,N-dimethylnitrosamine (25-200 mM) in th...
متن کاملDNA methylation by N-methyl-N-nitrosourea: methylation pattern changes in single- and double-stranded DNA, and in DNA with mismatched or bulged guanines.
The detection of abnormal DNA base pairing arrangements and conformations is chemically probed in synthetic 32P-end-labeled deoxyribonucleotide oligomers using N-methyl-N-nitrosourea (MNU) and 2,12,-dimethyl-3,7,11,17-tetraazabicyclo-[11.3.1]heptadeca-1 -[17],2,11,13,15 pentaene-Ni (II) (Ni-complex) with KHSO5. The DNA targets studied are single-stranded (s-s) DNA, double-stranded (d-s) DNA, d-...
متن کاملDNA base changes and alkylation following in vivo exposure of Escherichia coli to N-methyl-N-nitrosourea or N-ethyl-N-nitrosourea.
Dideoxy chain-termination DNA sequencing was used to determine the specific DNA base changes induced after in vivo exposure of Escherichia coli to N-methyl-N-nitrosourea (MNU) and N-ethyl-N-nitrosourea (ENU) using the xanthine guanine phosphoribosyltransferase (gpt) gene as the genetic target. The resultant mutation spectra were compared with the levels of O6-alkylguanine and O4-alkylthymidine ...
متن کاملSome effects of treatment of hen erythrocytes in vitro with N-methyl-N-nitrosourea.
Studies have been made of the effect of N-methyl-N-nitrosourea on hen erythrocytes in vitro. These were done to find whether the highly condensed state of the chromatin and the very low metabolic activity of these cells would affect the extent of methylation of the DNA and chromatin proteins and the persistence of any methylation sites in these macromolecules with time after treatment. Also, th...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1976
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/3.7.1689